Staining with propidium iodide was completed to tell apart dead from live cells. DNM1 Thermostability assay Cells were resuspended in FACS staining buffer for 10 min in room temperature accompanied by contact with 37, 45, 55, and 65 C for 10 min. in the PLC. Cells missing CRT exhibited decreased surface area MHC-I levels, in … Continue reading Staining with propidium iodide was completed to tell apart dead from live cells